Investigating the expression of GABAA receptor subunits in CDKL5 -/y rats to understand seizure mechanisms in CDKL5 Deficiency Disorder (CDD)

Could you briefly introduce yourself and your summer research project?

My name is Maisie Taylor, and I am currently in my fourth year studying Neuroscience. My summer research project focused on CDKL5 gene knockout (KO) mice, exploring how insights from rat models can improve our understanding of symptoms associated with CDKL5 deficiency disorder (CDD) in humans. In this project, I used immunohistochemistry and western blot analysis to investigate GABA receptor subunits in both wild-type and CDKL5 KO mice, aiming to understand the mechanisms underlying seizure generation and resistance.

What was the most rewarding part of your summer research?

The most rewarding aspect of this experience was applying my academic knowledge in a real-life laboratory setting. While learning and understanding lecture content is valuable, translating this knowledge into hands-on practice can deepen your comprehension and solidify key concepts. 

Why should students get involved in a summer research project?

Taking part in a summer research project allows you to demonstrate your skills to experts, network with researchers who will support your interests, and explore opportunities for further internships or employment. Most importantly, it also gives you the chance to gain experience in different lab techniques, helping to discover what you’re truly interested in pursuing as a career goal.

How has this research experience influenced your future study and career paths?

This experience has helped solidify my understanding of what it would be like to work in a laboratory, and what a future in this field could look like. From my internship, I have decided that the best path for me will be to complete a Master’s and eventually a PhD, which I may have been more unsure about before this experience. 

What advice would you give future students applying for summer research scholarships?

Trust in yourself and your abilities! While it may feel daunting at first, you already have the knowledge to succeed in the techniques you’re learning. Also, don’t be afraid to ask questions, as this is a crucial part of learning, growing, and deepening your understanding.