Investigating Maternal Heat Stress During Early Pregnancy: Optimisation of Immunohistochemistry in Porcine Endometrium

Could you briefly introduce yourself and your summer research project?

Hi, my name is Hannah. I am a Pharmacology BSc (Hons) student, entering my final year. This summer, I completed a four-week research project with the Forde laboratory group at the Centre for Reproductive Health, Institute for Regeneration and Repair, Little France.  The team investigates the impacts of heat stress on maternal and foetal health, using a porcine model. During my time, I aimed to optimise immunohistochemistry protocols for two markers of interest, Keratin 18 (KRT18) and Insulin-like Growth Factor Binding Protein 1 (IGFBP-1), to investigate potential changes in the maternal endometrium following heat stress. 

What was the most rewarding part of your summer research?

For me, the most rewarding part was successfully optimising the IHC protocol for both markers of interest. Knowing these protocols can be used for future experiments to save time and resources, and support further research is an amazing feeling. It's especially fulfilling to know my work can contribute to the group beyond my time in the lab. 

Why should students get involved in a summer research project?

It was a brilliant opportunity to gain exposure to hands-on experience and learn analytical techniques beyond the scope of my degrees. I also gained a better understanding of what working in a research environment is like. I think the experience will be invaluable for my upcoming fourth-year Research Project.

How has this research experience influenced your future study and career paths?

I really enjoyed meeting colleagues from different backgrounds and learning about the potential options available after graduation. I gained greater appreciation for the interdisciplinary nature of research and how everyone brings in their own skill set and knowledge to the team. The experience has also helped me understand better whether I could see myself pursuing a similar career after university.

What advice would you give future students applying for summer research scholarships?

Go for it! If you don’t ask, you won’t get. Don’t be afraid to reach out to researchers whose work interests you, even if you don’t have much previous experience. People are often very happy to help you with your career progression, and many of them have been in a similar position themselves at some point.