We cannot measure anything perfectly: our measurements always include some degree of uncertainty. This chapter explains how we can describe this uncertainty when reporting and interpreting results. Specifically, we introduce the idea of ‘standard error’ and ‘confidence intervals’. These concepts apply widely. Most obvious, you may be familiar figures that present mean values with error bars (usually standard error of the mean, or a 95% confidence interval). However, these concepts extend to statistical tests. For example, imagine we wished to test the null hypothesis that the average value of subjects in group ‘A’ equaled the average of subjects in group B (e.g, using a t-test; see Chapter, ‘Comparing averages’). In this example, we can think of the analysis a way of estimating the magnitude of the difference between the averages of the two groups in a way that accounts for uncertainty: the less uncertainty we have in our estimate of this difference, the greater our ability to judge whether the means of the two groups differ. In future chapters we’ll express and interpret this idea as ‘effect size’, and continue to use the concepts of ‘standard error’ and ‘confidence interval’ introduced in the present chapter.The main point is that measuring things in a way that accounts for uncertainty lies at the heart of both data presentation and analysis. This chapter introduces standard error and 95% confidence intervals as fundamental concepts in this light.Standard ErrorsA discussion and demonstration (based on simulations in R) of the basis and interpretation of standard errors. Document standard errors (128.99 KB / PPTX) 95% Confidence Interval: CalculationsHow to calculate a 95% confidence interval for a mean value of samples from a normally distributed population. Part 1 (of 3) of a discussion of confidence intervals Document Confidence intervals - part 1 (49.38 KB / PPTX) 95% Confidence Intervals: understanding their basisA discussion of the basis of 95% confidence intervals, focusing on why a standard error is multiplied by a number (e.g. 1.96). Part 2 (of 3) of a discussion of confidence intervals Document Confidence intervals part 2 (805.76 KB / PPTX) 95% Confidence Intervals: their interpretationA discussion of formal and less formal interpretations of 95% confidence intervals. Part 3 (of 3) of a discussion of confidence intervals Document Confidence intervals part 3 (150.84 KB / PPTX) Plotting means with with standard errors or confidence intervalsIn the chapter, ‘Plotting data’, you learned to plot data as boxplots and individual values. Sometimes you might wish to plot a mean value with error bars instead of using a boxplot. This file demonstrates how to create such plots in R for some types of data: Document Means and errors (417.46 KB / PDF) Practice problems and answers Document Experimental data chapter 6 Standard Error Confidence intervals Questions (78.35 KB / DOCX) Document Experimental data chapter 6 Standard Error Confidence intervals Answers (80.41 KB / DOCX) Recommended readingThe Analysis of Biological Data, see Chapter 'Estimating with uncertainty' by Whitlock and Schluter Review Quiz The attached Powerpoint presentation provides questions that review basic concepts from this chapter. Note that the questions sometimes present more than one correct answer, and sometimes all the options are incorrect! The point of these questions is to get you to think and to reinforce basic concepts from the videos. You can find the answers to the questions in the ‘notes’ section beneath each slide.Please view file ‘Quiz_MeasuringWithUncertainty’ Document Experimental data Quiz_Measuring With Uncertainty (143.66 KB / PPTX) This article was published on 2024-08-05