TIRF

IMPACT hosts a Total Internal Reflection Fluorescence (TIRF) microscope coupled to an Olympus IX81 epi-fluorescence microscope.

TIRF system at IMPACT is integrated onto a standard epi-fluorescence system from Olumpus IX81 microscope. This is a fully motorised microscope controlled by Olympus CellR software and is equipped with a Hamamatsu EMCCD camera.

Key features:

  • Suitable for TIRF imaging and/or standard epi-fluorescence imaging
  • Fully motorised, automatic filter/objective switching, time lase imaging, heated stage and CO2 incubator.
  • Fast image acquisition
Microscope Olympus IX81, POC cell cultivation system, Cage incubator
Objectives 10X UPLFLN/NA 0.3
  20X LUCPLFLN/NA 0.45
  60X PLAPO/TIRF/ NA 1.4 OIL
  150X UAPO/ TIRF/NA 1.45 OIL
Lasers 488nm, 561nm
Epi-Filter cubes 488/561 dual DAPI/FITC/TRITC CFP/YFP
Excitation Filter (MT20) 340 fura, 380 fura, 403 DAPI, 430 CFP, 492 FITC, 500 YFP, 572 TxRed
Camera EM CCD Hamamatsu
Applications TIRF/epi- fluorescence, fully automated multi-colour imaging, time series….
Software Cell^R

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