The specifications of the Nikon AXR-NSPARC High resolution imaging with galvano scanning mirrors (up to 8192 x 8192 pixels)High speed imaging with a resonant scanner-up to 2048 x 2048 pixelsHybrid scanning allows simultaneous use of Galvano and resonant scanners for simultaneous photo-activation and imaging66 channel spectral detectorSpectral unmixingTiled scan for large area image acquisitionJOBS and General Analysis 3 for automated image acquisition and analysis routines.Further informationMicroscopeNikon Ti2 E Inverted , OKOlab Cage incubatorObjectives4X Plan Flour/ NA 0.1 10X Plan Flour/ NA 0.3 20X Plan Apo VC/NA 0.8 25X CFI Plan Apo Lamda S Silicone 40X CFI Plan Apo Lamda S Silicone 60X Plan Apo VC/ NA 1.4 OILLasersLUD-S6 LaserUnit 405/445/488/514/561/638nmEpi-Filter cubesDAPI, CFP, GFP, Cherry, Cy5Standard detector2x MultiAlkaline PMTs, 2x GaAsP PMTsSpectral detector:2 tunable detectors from 400-920nm 66 channel sequential detection Precision unmixingScan head: Galvano:Pixel size: max. 8192 x 8192 pixels Resonant Scanner:Pixel size: max. 2048 x 2048 pixels Acquisition methodsStandard image acquisition/ High-speed image acquisition/ Simultaneous photo activation and image acquisitionApplicationsthree-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization, calcium imaging, FRAP, FRET, photo activation…Super-ResolutionNSPARC detector - filter based em 450/50, 525/50, 585/65, 700/75nmSoftwareNIS Elements (2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing, JOBS, GA3, Denoise.Ai, Clarity.Ai, deconvolution) This article was published on 2024-08-05
