Nikon A1R

The specifications of the Nikon A1R

High resolution imaging with galvano scanning mirrors (up to up to 4096 x 4096 pixels)
High speed imaging with a resonant scanner-up to 30 fps(512x512 pixels ) or 420 fps (512x32 pixels)
Hybrid scanning allows simultaneous use of Galvano and resonant scanners for simultaneous photo-activation and imaging
32 channel spectral detector
Spectral unmixing
Virtual Filter
Tiled scan for large area image acquisition
4096 grey intensity levels (12 bit images )

Microscope	Nikon Ti: E Inverted , POC cell cultivation system, Cage incubator
Objectives	4X Plan Flour/ NA 0.1
	10X Plan Flour/ NA 0.3
	20X Plan Apo VC/NA 0.8
	20X S Plan Flour ELWD/NA 0.5
	40X Plan Flour/ NA 1.3 OIL
	60X Plan Apo VC/ NA 1.4 OIL
	40X Plan Apo/ NA 0.95 (optional)
	40X Plan Apo/NA 1.25 OIL (optional)
	60X Plan Apo VC/ NA 1.20 Water (optional)
Lasers	405 nm, 488/504/514 nm, 561 nm, 633 nm
Epi-Filter cubes	DAPI, CFP, GFP, Cherry, Cy5
Standard detector	4 PMTs
Spectral detector:	32 channels 4 fps (256 x 256 pixels),
	1000 lps Wavelength range variable in 0.25nm steps High-speed unmixing,
	Precision unmixing
Scan head:
Galvano:	Pixel size: max. 4096 x 4096 pixels
	Scanning speed: 4 fps (512 x 512 pixels)
	Zoom: 1-1000x continuously variable
	Scanning mode: X-Y, XY rotation, Free line, Line Z
Resonant Scanner:	Pixel size: max. 512 x 512 pixels
	Scanning speed: 30 fps (512 x 512 pixels) to 230 fps (512 x 64 pixels)
	Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
	Scanning mode: X-Y, Line
Acquisition methods	Standard image acquisition/ High-speed image acquisition/ Simultaneous photo activation and image acquisition
Applications	three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization, calcium imaging, FRAP, FLIP, FRET, photo activation…
Additional Hardware	Bh TCSPC-FLIM (Fluorescence life time imaging module)
Software	NIS Elements (2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing)

This article was published on 2024-08-05