The specifications of the Nikon A1R High resolution imaging with galvano scanning mirrors (up to up to 4096 x 4096 pixels) High speed imaging with a resonant scanner-up to 30 fps(512x512 pixels ) or 420 fps (512x32 pixels) Hybrid scanning allows simultaneous use of Galvano and resonant scanners for simultaneous photo-activation and imaging 32 channel spectral detector Spectral unmixing Virtual Filter Tiled scan for large area image acquisition 4096 grey intensity levels (12 bit images ) Further information Microscope Nikon Ti: E Inverted , POC cell cultivation system, Cage incubator Objectives 4X Plan Flour/ NA 0.1 10X Plan Flour/ NA 0.3 20X Plan Apo VC/NA 0.8 20X S Plan Flour ELWD/NA 0.5 40X Plan Flour/ NA 1.3 OIL 60X Plan Apo VC/ NA 1.4 OIL 40X Plan Apo/ NA 0.95 (optional) 40X Plan Apo/NA 1.25 OIL (optional) 60X Plan Apo VC/ NA 1.20 Water (optional) Lasers 405 nm, 488/504/514 nm, 561 nm, 633 nm Epi-Filter cubes DAPI, CFP, GFP, Cherry, Cy5 Standard detector 4 PMTs Spectral detector: 32 channels 4 fps (256 x 256 pixels), 1000 lps Wavelength range variable in 0.25nm steps High-speed unmixing, Precision unmixing Scan head: Galvano: Pixel size: max. 4096 x 4096 pixels Scanning speed: 4 fps (512 x 512 pixels) Zoom: 1-1000x continuously variable Scanning mode: X-Y, XY rotation, Free line, Line Z Resonant Scanner: Pixel size: max. 512 x 512 pixels Scanning speed: 30 fps (512 x 512 pixels) to 230 fps (512 x 64 pixels) Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x) Scanning mode: X-Y, Line Acquisition methods Standard image acquisition/ High-speed image acquisition/ Simultaneous photo activation and image acquisition Applications three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization, calcium imaging, FRAP, FLIP, FRET, photo activation… Additional Hardware Bh TCSPC-FLIM (Fluorescence life time imaging module) Software NIS Elements (2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing) This article was published on 2024-08-05
