IMPACT has two stand-alone Zeiss LSM510 confocal systems, both are coupled to a Ti: Sapphire laser for two-photon fluorescence imaging. Since two-photon fluoresce happens only at the focus, no confocal pin-hole is needed. This improves the signal to noise ratio of the image as well as reduces the out of focus photobleaching. The use of near infra-red laser has the benefit of being able to penetrate deeper into the tissue than visible light. However, due to the longer excitation laser wavelength the optical resolution along the axis is worse compared to a standard confocal system. Objects obscured by background fluorescence may appear brighter or more defined in multi-photon image. IMPACT has two stand-alone Zeiss LSM510 confocal systems (one inverted and one upright) both are coupled to a Coherent Mira mode-locked Ti:Sapphire laser operating at 80MHz, ~200fs pulses tuneable over a wavelength range of 780-880 nm. Email IMPACT for more details This article was published on 2024-08-05
