Dr Vinothini Prabhakaran and Dr Jennifer Paxton have recently published a new paper in the journal Organoids. This study investigated the formation of enthesis-like (bone-tendon) structures formed through the triphasic co-culture of bone, tendon and bone mesenchymal stem cells.This model will be used to further explore the processes of enthesis formation and repair in vitro to make meaningful progress in orthopaedic injuries and disease.To read the full paper, click here. Microscopic images of H&E-stained sections of triphasic constructs and toluidine blue stained sections of (C) biphasic and (D) triphasic constructs cultured in growth media (control) and chondrogenic media (ChM) on day 4 and day 20). Scale bars: whole constructs—500 µm, magnified interface regions—200 µm. ‘B’ denotes bone region with dROb spheroids; ‘BM’ denotes interface region with BMSC spheroids, ‘T’ denotes tendon region with tendon spheroids. H&E staining shows blue nuclei and pink cytoplasm; Toluidine blue staining shows blue nuclei and purple metachromatic sulphated proteoglycans. Publication date 19 Feb, 2026
